Composite

Part:BBa_K1683004:Design

Designed by: Matthew W Mortensen   Group: iGEM15_WLC-Milwaukee   (2015-09-17)


pBAD+Strong RBS+E. coli tolC signal sequence+P. aeruginosa oprM


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
    Illegal NheI site found at 146
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 253
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

BBa_K206000 was chosen for its ability to be controlled with a common inducer (arabinose) as well as its high rate of transcription. BBa_B0034 was chosen for its high translation efficiency. The main consideration when designing the signaling sequence and Pseudomonas aeruginosa PA01 TolC gene was to use silent mutations alter any RFC10 incompatible DNA sections to be biobrick compatible.


Source

BBa_K1406000 comes from the biobrick registry The tolC signaling sequence and Pseudomonas aeruginosa PA01 tolC sequence were synthesized from DNA sequences found in genomic databases.

References